his study demonstrates the first successful application of phase retrieval holography to microscopy. We developed a dual-camera phase retrieval holographic microscopy system that captures two in-line holograms at different axial positions. This system retrieves quantitative phase distributions through iterative reconstruction and mitigates the twin image problem inherent in conventional in-line holography. The relative error in measuring the volume of a 500 µm-diameter ball lens was significantly reduced from 95% with the conventional method to 3.9% with the proposed method. These results confirm the feasibility and effectiveness of the proposed approach for precise, phase-based measurements in microscopic imaging.
